Keothavong claimed the opening set of the second round tie before the German fought back to win 4-6 6-3 6-3.
The Briton saw off Hungarian qualifier Melinda Czink in the first round, but could not maintain an impressive start against world number 16 Kerber.
The 24-year-old, who won her maiden WTA event in Paris earlier this year, faces compatriot Mona Barthel in round three.
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